Arginine-C™, Sequencing Grade, G-Biosciences

Supplier: G-Biosciences

786-11 786-11

CA82022-502PK 4950.68 CAD

CA82022-502 82022-502

Arginine-C™, Sequencing Grade, G-Biosciences

Nucleic Acid Reagents Sanger Sequencing Reagents

SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

SG-Arginine-C™ is a modified Arginine-C with high specificity, free from other known endopeptidases, that is resistant to autolysis and degradation. SG-Chymotrypsin™ is used for the digestion of proteins for sequence analysis and is suitable for sequencing applications. Use of SG-Arginine-C™ produces consistent activity levels from lot to lot for reproducible digestions.

SG-Arginine-C™ is supplied lyophilized in an activated form in 5μg vials and can be reconstituted to a concentration of 0.25μg/mL by addition of 20μL per vial of the supplied reaction buffer. For fragmentation the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight).

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