VWR® SOB Broth, Biotechnology Grade

Supplier: Avantor

VWR® SOB Broth, Biotechnology Grade

VWR® SOB Broth, Biotechnology Grade

VWR®

VWRVJ906-500G J906-100G

97063-454CS 1859.61 CAD

97063-452 97063-454

VWR® SOB Broth, Biotechnology Grade

Media for Microbiology Dehydrated Media for Microbiology

SOB broth powder is used for the preparation of nutritionally rich growth medium and transformation of competent <italic>E

SOB Broth (Super Optimal Broth) growth medium is nutritionally rich, containing casein hydrolysate and yeast extract as sources of nitrogenous compounds and growth factors,and magnesium sulfate as a supply of magnesium ions necessary for DNA replication.

when dissolved at 25.5 g per liter of water,yields the formulation
Tryptone -------- 20 Grams/L
Yeast Extract- - -5 Grams/L
Sodium Chloride--0.5 Gram/L

Medium should be autoclaved or sterile-filtered. Allow the solution to cool to 50 °C or below before adding supplemental components and antibiotic. Add 20 mL of 1 M MgSO4.Store SOB Broth powder at room temperature (18 – 26 °C). Store prepared medium cold (2 – 8 °C).

coli.</italic>

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