HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid, high purity

Supplier: VWR

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidN-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acidHEPES free acid

VWRV0511-1KG VWRV0511-50G VWRV0511-250G
97061-826CS 1329.73 CAD
97061-826 97061-822 97061-824
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid, high purity
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.


Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Melting Pt: 234 °C
Density: 1.448 g/cm³ (20 °C)
MDL Number: MFCD00006158
CAS Number: 7365-45-9
Merck Index: 13,04672
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Specification Test Results

DNase none detected
Heavy Metals (as Pb) = 0.0005 %
Iron = 0.0005 %
pKa @20°C 7.45 - 7.65
Protease none detected
Purity (Dry Basis) 99 %
Residue on Ignition 0.2 %
RNase none detected

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