HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid, high purity
Supplier: VWR
Synonyms:
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid
, 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid
, HEPES free acid
One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.
Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
Formula:
C₈H₁₈N₂O₄S MW: 238.31 g/mol Melting Pt: 234 °C Density: 1.448 g/cm³ (20 °C) |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 Merck Index: 13,04672 |
Specification Test Results
DNase | none detected |
Heavy Metals (as Pb) |
|
Iron |
|
pKa @20°C | 7.45 - 7.65 |
Protease | none detected |
Purity (Dry Basis) |
|
Residue on Ignition |
|
RNase | none detected |
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