E.Z.N.A.® Plasmid Isolation Kits, Omega Bio-Tek
Supplier: OMEGA BIO-TEK CA
The E.Z.N.A.® plasmid systems are able to isolate plasmid DNA by means of HiBind™ columns, Mag-Bind™ beads, or isopropanol precipitation
The endotoxin-free mini kits can purify up to 30µg of DNA from 1–5mL (Mini I) or 10–15mL (Mini II) culture sizes in 45 minutes. The endotoxin-free midi kits can purify up to 200µg of DNA from 30–50mL of overnight culture in 60 minutes. The endotoxin-free maxi kits can purify up to 1mg of DNA from 100–200mL of overnight culture in 60 minutes.
The E.Z.N.A.® method allows for plasmid DNA isolation from bacteria, yeast, or M13 phage. Plasmid, BAC, PAC, YAC, P1, cosmid, and phage DNA can be isolated and used for restriction digestion, transfection, transformation, routine screening, and other downstream applications. V-spin columns feature an attached cap while Q-spin and Mini II columns are capless. V-spin, Q-spin, Mini II, Midi, and Maxi columns have a vacuum luer tip for a leak-free connection to a vacuum manifold or luer hub needle. These columns can be used for both centrifugation and vacuum protocols. Fastfilter® kits allow midi- and maxi-scale plasmid isolation in less than 40 minutes.
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