Fast-Ion Maxi Plasmid Kits, IBI Scientific

Supplier: IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Fast-Ion Maxi Plasmid Kits, IBI Scientific

IB47120 IB47121 IB47122

95039-660EA 173.63 CAD

95039-660 95039-662 95039-664

Fast-Ion Maxi Plasmid Kits, IBI Scientific

Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents

IBI’s Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures

Sample Size: 100ml high-copy plasmid/250ml low-copy plasmid
Operation: Gravity Flow Column
Expectant Yield: 500µg to 1mg of plasmid DNA
Operation Time: 120 minutes or less

The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

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