Gram′s safranin solution for Gram staining, Sigma-Aldrich®
Supplier: MilliporeSigma
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Warning
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Synonyms:
Safranin T solution
M-Clarity™ quality level = MQ400
This ready-to-use Gram's safranine solution-for the Gram staining method, is used in bacteriology.
The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear pink to red.
A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no.1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
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Formula:
C₂₀H₁₉ClN₄ MW: 350.85 g/mol Storage Temperature: Room Temperature |
MDL Number:
MFCD00011759 CAS Number: 477-73-6 |
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