The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR + pan bacteria) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.
- Ideal for analysis of samples containing complex mixtures of eukaryotic and bacterial rRNA
- 3 to 7-fold increase in coverage of SARS-CoV-2 and other genomes in nasopharyngeal (NSP) samples
- Single workflow to detect viral genomic data, microbiome composition, co-infections, and host gene expression
- Streamlined Jumpcode™ CRISPRclean workflow: stranded total RNA library prep with ribodepletion
Improve the discovery of high-value and biologically interesting transcripts. Powered by CRISPR-Cas9, ribodepletion is highly efficient and programmed to cut ribosomal RNA from human sequences (5S, 5.8S, 18S, 28S, 45S (precursor), mitochondrial 12S and 16S sequences), and 212 bacterial species from all phyla (5S, 16S, and 23S rRNA sequences). A tested method is available to automate jumpcode genomics CRISPRclean plus stranded total RNA prep with rRNA depletion (human, mouse, rat, pan bacteria) kit on the Sciclone™ G3 NGSx iQ™ liquid handler. The NEXTFLEX™ RNA-seq 2.0 unique dual index barcodes (1-384) are provided at a concentration optimized for use with jumpcode genomics CRISPRclean plus stranded total RNA prep with rRNA depletion (human, mouse, rat, pan bacteria) kit.
Certifications: Manufactured in ISO 9001 facility.
Caution: For research use only. Not for use in diagnostic procedures.