The DepleteX® single cell RNA boost kit employs a CRISPR-based method to selectively remove unwanted reads.
- Enhance secondary analysis with ~50% more useful reads
- Verified for both short-read and long-read sequencing
- Efficiently removes ribosomal, mitochondrial, non-transcriptomic reads, and optionally non-variable genes from single-cell libraries
- Streamlined 3-step protocol
- Tested with 10× genomics single cell sequencing and other scRNA sequencing workflows
Enhance usable single-cell RNA-seq data using DepleteX ® single cell RNA boost kit. DepleteX ® efficiently removes abundant and uninformative fragments before sequencing, reallocating 50% of sequencing clusters to informative reads that would typically be filtered out during secondary analysis. This kit utilizes Cas9 depletion with an optimized guide set to target ribosomal and mitochondrial mRNA, non-transcriptomic reads, and highly expressed non-variable genes. Additionally, the kit provides a separate tube for non-variable gene depletion, which can be included or excluded as required.
The DepleteX® single cell RNA boost kit empowers users to reduce noise and optimize usable data in single-cell RNA sequencing, and reduces costs associated with single cell sequencing. Implementing single-cell sequencing can be costly. However, the DepleteX® single cell RNA boost kit provides cost savings by efficiently eliminating abundant and uninformative fragments before sequencing, reallocating 50% of sequencing clusters to informative reads that would typically be filtered out during secondary analysis.
Certifications: Manufactured in ISO 9001 facility.
Caution: For research use only. Not for use in diagnostic procedures.
