The DepleteX® nasopharyngeal microbial RNA boost kit selectively removes highly expressed transcripts in vivo before sequencing.
- Efficient human RNA depletion from nasopharyngeal samples
- Enhanced pathogen detection in challenging nasopharyngeal samples with significant human RNA presence
- Facilitates virus detection at high Cq values (up to 30) using 4 million reads
Enhance microbial discovery by reducing abundant human reads. Nasopharyngeal RNA samples often exhibit low quality and quantity due to dominance by host and commensal microbial RNA. This combination of factors - low input, poor quality RNA, and high host contamination - reduces sensitivity in metagenomic next - generation sequencing (mNGS) and hinders the detection of low-expressing microbes and novel pathogens.
The DepleteX® nasopharyngeal microbial RNA boost kit is used during the RNA-seq library prep workflow starting with nasopharyngeal samples after the PCR step and then Post-depletion PCR amplification ensures that only uncleaved fragments, i.e., those containing sequences of interest, are amplified further. A final bead-based cleanup step ensures that the library is ready for sequencing. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX® rapid directional RNA-seq kit 2.0.
Certifications: Manufactured in ISO 9001 facility.
Caution: For research use only. Not for use in diagnostic procedures.
