PureRec Duplex-Specific Nuclease
Supplier: Zymo Research
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
- Highest specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA
- Premium purity: RNase-free, protease-free, and animal-free
- Thermostable: Able to withstand high temperatures without denaturation or loss in activity
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg²⁺ ions, with a recommended concentration of 5 mM for most applications. PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
Applications:
Remove gDNA and DNA in DNA:RNA hybrids for protein or RNA preparations
Reduce abundant rRNA and globin transcripts to enhance next-generation sequencing (NGS) libraries
Achieve precise single-nucleotide polymorphism (SNP) detection with minimal off-target effects
Enhance signal amplification for accurate virus detection
Improve detection of miRNA and biomarkers
Optimal temperature: 60 °C
Reaction buffer: 10X DSN digestion buffer 500 mM Tris-HCl, 50 mM MgCl₂, pH 8.0
Stop solution: 2X DSN stop solution 10 mM EDTA, pH 8.0
Unit definition: One Kunitz unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25 °C, pH 7.15, when acting on calf thymus DNA.
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