Labeling Reagent, Cytiva

Supplier: Cytiva

Labeling reagent

Cell proliferation biotrak ELISA

Cell proliferation Biotrak ELISA

Anti-BrdU antibody

Labeling reagent

Cell proliferation biotrak ELISA

Cell proliferation Biotrak ELISA

Anti-BrdU antibody

Biotrak™

RPN201 RPN250 RPN201

CA95019-026LEA 630.06 CAD

CA95019-026L CA95019-106L 95019-026

Labeling Reagent, Cytiva

Assays Immunological Assays

The Cell Proliferation Kit is an ICC system for monitoring cell proliferation using monoclonal anti-5-bromo-2'-deoxyuridine (BrdU) to detect BrdU incorporated into cellular DNA.

Non radioactive
High level of correlation with the number of proliferating cells, with low mean variation
As least as sensitive as ³H-thymidine incorporation
Immunoassay can be performed in ∼3 hours
Use of a multiwell ELISA reader allows a large number of samples to be processed simultaneously

Used in conjunction with RPN20, the reagent allows extended use of the kit where additional amounts of labeling reagent are required, such as in vivo studies.

The antibody contains a nuclease to produce single stranded DNA without the need for chemical denaturation. Cellular morphology is preserved allowing multiple labeling experiments to be carried out. The high-contrast stain produced has sensitivity comparable to autoradiographic methods.

Ordering information: Additional labeling reagent and antibody for use with the immunocytochemistry kit are available separately. NOTE: DAB component must be ordered separately.

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