IEF/SDS Sample Application Strips, Cytiva

Supplier: Cytiva

IEF/SDS Sample Application Strips, Cytiva

IEF/SDS Sample Application Strips, Cytiva

CleanGel™

18103532 18-1002-26 18-1035-32 18-1002-26

CA95017-241LPK 1381.3 CAD

95017-241 95017-197 CA95017-241L CA95017-197

IEF/SDS Sample Application Strips, Cytiva

Electrophoresis Gels Precast Gels

CleanGel IEF is a dried polyacrylamide gel optimised for analytical isoelectric focusing (IEF). The gel is excellent for separating enzymes with preserved activity.

Perform sensitive procedures using precast gels that are carefully washed and dried after casting to remove catalysts and unpolymerised material
Customise IEF separations by reswelling in a suitable mixture of carrier ampholytes and other additives such as detergents or denaturants
Handle mechanically stable, plastic-backed gels with confidence

The gel is cast on a polyester support film and carefully washed to remove catalysts and nonpolymerized substances and then dried. Prior to isoelectric focusing, the dried gel is rehydrated in a solution containing Pharmalyte™ carrier ampholytes. Additives such as urea and/or detergents can also be added at this stage. CleanGel IEF is rehydrated in a flat tray (GelPool) to a thickness of 0,43 mm.

Introducing carrier ampholytes and additives into the gel after the polymerisation eliminates the risk of copolymerising chemicals in the gel matrix. Thus, CleanGel IEF gels give low electroendosmosis, resulting in sharp bands and minimum pH gradient drift. Even additives that are sensitive to polymerisation reactions, or that inhibit the polymerisation can be used.

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