NF[kappa]B Transcription Factor Assay Kits, Rockland Immunochemicals
Supplier: Rockland Immunochemical
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates
A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
Ordering information: Kits contain transcription factor A, NF[kappa]B p50 or NF[kappa]B p65 positive control and primary antibody, HRP-conjugated goat anti-rabbit secondary antibody, specific competitor dsDNA, antibody binding buffer (10X), binding assay (4X), wash buffer concentrate (400X), Tween®-20 polysorbate, developing solution, stop solution, and 96-well plate and plate cover.
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