EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer superior performance, convenience, reliability, and value for routine PCR
EconoTaq® PLUS master mixes are ready to use and contain Mg++ at an optimized level. Both master mixes contain dNTPs and PCR Enhancer, and EconoTaq PLUS GREEN also contains tracking dyes.
EconoTaq mix is more effective. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes. High density reaction buffer enables direct gel loading following PCR. Excellent routine PCR results. EconoTaq PLUS GREEN and EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb. EconoTaq can amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98°C to amplify challenging GC-rich templates, while others cannot. EconoTaq PLUS GREEN contains convenient tracking dyes. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp. If desired, the dyes are easily removed by standard DNA purification products or ethanol precipitation. For fluorescence or absorbance detection assays, EconoTaq PLUS 2X Master Mix offers the same great performance without the dyes. Flexibility and ease of use. EconoTaq PLUS GREEN and EconoTaq PLUS are packaged in 50 reaction vials that can be stored in the refrigerator (+4ºC) for up to 3 months. They are stable for at least 10 freeze-thaw cycles.
PCR Activity: EconoTaq PLUS GREEN and EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers. Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA. Absence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis. Absence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels. Purity: EconoTaq DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination. The nucleotides in the Master Mix are certified free of nucleases and phosphatases.
For more challenging PCR these master mixes can also be cycled up to 98°C to denature GC-rich and other difficult templates.
Ordering information: Use EconoTaq PLUS GREEN 2X Master Mix when the PCR products will be analyzed by agarose gel electrophoresis and ethidium bromide staining. Use EconoTaq PLUS 2X Master Mix when the PCR products will be analyzed by absorbance or fluorescence excitation, to avoid possible interference by the dyes. EconoTaq PLUS GREEN & EconoTaq PLUS prices are based on a 50 µl PCR reaction.
