bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC), Fluorochrome fluorescent dye, Calbiochem®

Supplier: MilliporeSigma

Synonyms: 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride, BBIH, HOE 33258, Bisbenzimide, 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole trihydrochloride, Hoechst 33258

382061-100MG 382061-1GM 382061-250MG
CA80056-700EA 375.3 CAD
CA80056-700 CA80056-702 CA80056-704
bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC), Fluorochrome fluorescent dye, Calbiochem®
bisBenzimide H 33258 trihydrochloride (Hoechst 33258)

Membrane-permeable, adenine-thymine-specific fluorescent stain


Excitation maximum: 346nm. Emission maximum: 460nm.


Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (80056-706, -710, and -708) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs.

Formula: C₂₅H₂₄N₆O·3HCl
MW: 533.88 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00012679
CAS Number: 23491-45-4

Order Now


Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR