bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC), Fluorochrome fluorescent dye, Calbiochem®
Supplier: MilliporeSigma
Synonyms:
4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride, BBIH, HOE 33258, Bisbenzimide, 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole trihydrochloride, Hoechst 33258
Membrane-permeable, adenine-thymine-specific fluorescent stain
Excitation maximum: 346nm. Emission maximum: 460nm.
Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (80056-706, -710, and -708) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs.
Formula:
C₂₅H₂₄N₆O·3HCl MW: 533.88 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00012679 CAS Number: 23491-45-4 |
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