The Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.
- Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
- Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
- Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
- Rapid—stain in less than 30 minutes; completely erase and reverse staining in less than 15 minutes
- Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps
This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on PVDF membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.
The Pierce Reversible Protein Stain Kit for PVDF Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for PVDF membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.
The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.
Contents
Sufficient For: 10 PVDF membranes (8 cm x 8 cm) from mini gels (SDS-PAGE)
• Sensitizer, 250 mL
• Reversible Stain, 250 mL
• Destain, 1 L
• Stain Eraser, 250 mL
Caution: Store at room temperature. For Research Use Only. Not for use in diagnostic procedures.
