Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), Ultrapure Bioreagent for molecular biology, J.T.Baker®

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Synonyms: Trizma(R) baseTRIS baseTHAM2-Amino-2-(hydroxymethyl)-1,3-propanediolTris(hydroxymethyl)methylamine2-Amino-2-(hydroxymethyl)propane-1,3-diol2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris)Tromethamine

Management of Change (MOC) category = NI

BAKR4109-09 4109-07 4109-F7 BAKR4109-01 4109-06 BAKR4109-F7 4109-02 4109-09 BAKR4109-06 BAKR4109-07 4109-01 BAKR4109-02
CAJT4109-1EA 353.15 CAD
CAJT4109-1 JT4109-2 JT4109-1 CAJT4109-2 JT4109-F7 CAJT4109-6 CAJT4109-7 CAJT4109-9 CAJT4109-F7 JT4109-09 JT4109-7 JT4109-6
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), Ultrapure Bioreagent for molecular biology, J.T.Baker®
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Buffer for molecular biology and liquid chromatography.


  • Lot analysis on label


Material also meets the chemical specifications of Tromethamine, USP.

Formula: NH₂C(CH₂OH)₃
MW: 121.14 g/mol
Boiling Pt: 357 °C (1013 hPa)
Melting Pt: 169…172 °C
Density: 1.328 g/cm³ (20 °C)
MDL Number: MFCD00004679
CAS Number: 77-86-1
Merck Index: 13,09842
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Specification Test Results

For Laboratory,Research,or Manufacturing Use
Assay (C₄H₁₁NO₃) (dried basis) ≥99.9 %
Assay (C₄H₁₁NO₃) 99.0 - 101.0 %
Appearance (White crystals) Passes Test
Absorbance of a 1M Solution - 260 nm ≤0.06
Absorbance of a 1M Solution - 280 nm ≤0.06
Absorbance of a 1M Solution - 400 nm ≤0.01
RNase Activity (None Detected) Passes Test
DNase Activity (None Detected) Passes Test
Protease Activity (None Detected) Passes Test
pH of 0.1M Solution at 25°C 10.0 - 11.0
Insoluble Matter ≤0.005 %
Water (H₂O)(by Karl Fischer titrn) ≤0.3 %
Trace Impurities - Arsenic (As) ≤1 ppm
Trace Impurities - Calcium (Ca) ≤1 ppm
Trace Impurities - Copper (Cu) ≤1 ppm
Trace Impurities - Iron (Fe) ≤1 ppm
Trace Impurities - Lead (Pb) ≤1 ppm
Trace Impurities - Magnesium (Mg) ≤1.000 ppm
Trace Impurities - Manganese (Mn) ≤1 ppm
Identification A Passes Test
Identification B Passes Test
Identification C Passes Test
Description Passes Test
pH (1 in 20) 10.0 - 11.5
Loss on Drying ≤1.0 %
Residue after Ignition ≤0.10 %
Heavy Metals (as Pb) ≤0.001 %
Melting Range(Lower) 168 - 172 °C
Melting Range(Upper) 168 - 172 °C
No Class 1,2,3 or other solvents are used or produced in the
manufacturing or purification of the product.
Material also meets the chemical specifications of Tromethamine, U.S.P.
Manufactured exclusively for Avantor Performance Materials, LLC., by BioSpectra, Inc., Stroudsburg, PA

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