Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), Ultrapure Bioreagent for molecular biology, J.T.Baker®
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
Synonyms:
Trizma(R) base
, TRIS base
, THAM
, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
, Tris(hydroxymethyl)methylamine
, 2-Amino-2-(hydroxymethyl)propane-1,3-diol
, 2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris)
, Tromethamine
Management of Change (MOC) category = NI
Buffer for molecular biology and liquid chromatography.
- Lot analysis on label
Material also meets the chemical specifications of Tromethamine, USP.
Formula:
NH₂C(CH₂OH)₃ MW: 121.14 g/mol Boiling Pt: 357 °C (1013 hPa) Melting Pt: 169…172 °C Density: 1.328 g/cm³ (20 °C) |
MDL Number:
MFCD00004679 CAS Number: 77-86-1 Merck Index: 13,09842 |
Specification Test Results
For Laboratory,Research,or Manufacturing Use | |
Assay (C₄H₁₁NO₃) (dried basis) | ≥99.9 % |
Assay (C₄H₁₁NO₃) | 99.0 - 101.0 % |
Appearance (White crystals) | Passes Test |
Absorbance of a 1M Solution - 260 nm | ≤0.06 |
Absorbance of a 1M Solution - 280 nm | ≤0.06 |
Absorbance of a 1M Solution - 400 nm | ≤0.01 |
RNase Activity (None Detected) | Passes Test |
DNase Activity (None Detected) | Passes Test |
Protease Activity (None Detected) | Passes Test |
pH of 0.1M Solution at 25°C | 10.0 - 11.0 |
Insoluble Matter | ≤0.005 % |
Water (H₂O)(by Karl Fischer titrn) | ≤0.3 % |
Trace Impurities - Arsenic (As) | ≤1 ppm |
Trace Impurities - Calcium (Ca) | ≤1 ppm |
Trace Impurities - Copper (Cu) | ≤1 ppm |
Trace Impurities - Iron (Fe) | ≤1 ppm |
Trace Impurities - Lead (Pb) | ≤1 ppm |
Trace Impurities - Magnesium (Mg) | ≤1.000 ppm |
Trace Impurities - Manganese (Mn) | ≤1 ppm |
Identification A | Passes Test |
Identification B | Passes Test |
Identification C | Passes Test |
Description | Passes Test |
pH (1 in 20) | 10.0 - 11.5 |
Loss on Drying | ≤1.0 % |
Residue after Ignition | ≤0.10 % |
Heavy Metals (as Pb) | ≤0.001 % |
Melting Range(Lower) | 168 - 172 °C |
Melting Range(Upper) | 168 - 172 °C |
No Class 1,2,3 or other solvents are used or produced in the | |
manufacturing or purification of the product. | |
Material also meets the chemical specifications of Tromethamine, U.S.P. | |
Manufactured exclusively for Avantor Performance Materials, LLC., by BioSpectra, Inc., Stroudsburg, PA |
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