NEBNext® Enzymatic 5hmC-seq Kits

Supplier: New England Biolabs
NEBNext®
E3350L E3350S E3350L E3350S
CA77441-292EA 4510 CAD
77441-292 CA77441-292 77441-294 CA77441-294
NEBNext® Enzymatic 5hmC-seq Kits
Nucleic Acid Reagents Next Generation Sequencing Reagents
The NEBNext® enzymatic 5hmC-seq kit specifically detects 5hmC sites.

  • 5hmC detection at single base resolution
  • Enzyme-based conversion minimizes DNA damage
  • 0.1 to 200 ng input range
  • Even GC coverage
  • E5hmC-seq and EM-seq data can be combined

The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.
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