Nucleofector® P4 Primary Cell 4D-X Kit L
Supplier: LONZA PHARMA - BIOSCIENCE CA
The P4 primary cell 4D-Nucleofector® X kit L is one of our five 4D-Nucleofector® Kits suited for transfecting primary cells in the 100 µl Nucleocuvette® vessel format when working with the 4D-Nucleofector® X unit.
- Benefits:
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
This kit has been determined to be optimal for transfecting following cell types:
B cell (mouse)
DC (mouse), immat.
MEF (mouse)
Applications:
Transfection of cell numbers in the range of 2×10⁵ to 2×10⁷ cells.
Low throughput applications.
The kit is available in different variants for either 12 or 24 reactions.
Delivery information: CA10064-516 includes 2×0.675 ml P1 primary cell Nucleofector® solution, 2×0.15 ml supplement 1, 50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0), 12×100 µl Nucleocuvette® vessel, 12×single use pipettes.
CA10064-518 includes 2.25 ml P1 Primary Cell Nucleofector® solution, 0.5 ml supplement 1, 50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0), 24×100 µl Nucleocuvette® vessel, 24×single use pipettes.
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