HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

Supplier: ReVacc Scientific

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

U90R79-100UG U90R79-500UG

77394-722EA 1168.41 CAD

77394-722 77394-724

HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

Proteins and Peptides

A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from BG505 strain (clade A, GenBank: ABA61516.1) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein (end at D664) with furin cleavage site replaced by a linker, a T332N mutation for glycan, a I559P mutation for stable trimer formation, and C-terminal 8 hexa-histidine tag.

This product has been evaluated by Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE gel for purity and ELISAs for binding, see results. Due to the unique feature of BG505 at residue 241 glycan hole, a rabbit antibody 11B can target this protein (ref: Cell Rep. 2016;16(9):2327-38.), but not other HIV GP with 241N, such as YU2 or 16055 trimer.

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