The Primary cell optimization 4D-Nucleofector® X kit is the ideal tool to conveniently and rapidly determine nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X unit.
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined in the 16-well Nucleocuvette® Strips are transferable to the 100 μl single Nucleocuvette® in the 4D-Nucleofector® X Unit
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
With the unique capability of the 4D-Nucleofector® X Unit to address individual wells of a 16-well Nucleocuvette® Strip with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using five 16-well Nucleocuvette® Strips and our pmaxGFP® Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette® Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs.
Basic Protocols are available for following cell groups:
Endothelial cells (mammalian)
Epithelial cells (mammalian)
Fibroblasts (mammalian)
Glial cells (mammalian)
Neurons (mammalian)
Smooth muscle cells (mammalian)
Stem cells (human)
Applications:
Determination of nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
Delivery information: Content:
0.675 ml Nucleofector® Solution P1.
0.675 ml Nucleofector® Solution P2.
0.675 ml Nucleofector® Solution P3.
0.675 ml Nucleofector® Solution P4.
0.675 ml Nucleofector® Solution P5.
4×0.15 ml Supplement 1.
1×0.15 ml Supplement 3.
50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0).
6×20 µl 16-well Nucleocuvette® strips.
