Nucleofector® Cell Line Optimization 4D-X Kit

Supplier: LONZA PHARMA - BIOSCIENCE CA

V4XC-9064
CA10064-228EA 1789.35 CAD
CA10064-228
Nucleofector® Cell Line Optimization 4D-X Kit
Transfection Reagents

The cell line optimization 4D-Nucleofector® X kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® X unit.


  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined in the 16-well Nucleocuvette® Strips are transferable to the 100 μl single Nucleocuvette® in the 4D-Nucleofector® X Unit
  • Conditions are transferable between 4D Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System


With the unique capability of the 4D-Nucleofector® X Unit to address individual wells of a 16-well Nucleocuvette® Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well Nucleocuvette® Strips and our pmaxGFP® Vector as positive control, our three Cell Line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette® Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.


Applications: Determination of nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® X unit.


Delivery information: Content:
0.675 ml Nucleofector® Solution SE.
0.675 ml Nucleofector® Solution SF.
0.675 ml Nucleofector® Solution SG.
3×0.15 ml Supplement 1.
50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0).
4×20 µl 16-well Nucleocuvette® strips.

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