ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kits
Supplier: LONZA PHARMA - BIOSCIENCE CA
ViaLight® Plus BioAssay kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability.
- Fast - Results from a 96 well plate can be processed and analyzed in as little as 10 minutes
- Sensitive - Detect as few as 10 cells allowing for lower seeding densities and more assays
- Convenient - Simply add two reagents directly to your culture well and read. No cell washing or medium removal required
- Flexible - Dynamic range of 5 decades and excellent performance with both adherent or suspension cultures allows you to design the experiment for your needs
- Robust - Stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing, and unlike MTT or Alamar Blue assays, ViaLight® Bioassays are not prone to interference due to culture condition changes
Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture.
The ViaLight® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers.
Delivery information: Contents:
500 tests (Sufficient for 5 plates) 1 × LT27-212 ATP monitoring reagent plus, lyophilized, 1 × LT27-079 assay buffer (50 ml bottle), 1 × LT27-076 Cell lysis reagent (50 ml bottle).
1000 tests (Sufficient for 10 plates) 2 × LT27-212 ATP monitoring reagent plus, lyophilized, 2 × LT27-079 Assay buffer (50 ml bottles), 1 × LT27-076 Cell lysis reagent (50 ml bottle).
10000 tests (Sufficient for 100 plates) 10 × LT27-213 ATP monitoring reagent plus, lyophilized, 10 × LT27-080 Assay buffer (100 ml bottles), 5 × LT27-077 Cell lysis reagent, 100 mL bottles.
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