Human Complex IV ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complex IV in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 100 - 1600 ng/L
- Sensitivity: 7.49 ng/L
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Complex IV ELISA kit (A313968) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Complex IV in serum, plasma or other biological fluids. An antibody specific for Complex IV has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complex IV present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Complex IV antibody, which also binds the Complex IV present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Complex IV antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Complex IV antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Complex IV captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Complex IV in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, steptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.
