Human Cell Adhesion Molecule 4 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Cell Adhesion Molecule 4 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human cell adhesion molecule 4 ELISA kit (A313406) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human cell adhesion molecule 4 in serum, plasma or other biological fluids. An antibody specific for cell adhesion molecule 4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the cell adhesion molecule 4 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Cell adhesion molecule 4 antibody, which also binds the cell adhesion molecule 4 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Cell adhesion molecule 4 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Cell adhesion molecule 4 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of cell adhesion molecule 4 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of cell adhesion molecule 4 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.
