This kit is developed for serologic test for bovine or sheep IgG titer of Anti-Brucella antibody in serum/plasma or purified bovine or sheep antibody (monoclonal and polyclonal) in vitro.
- This kit was tested to bind to convalescent sheep sera (n = 4) with sensitivity of 100%, specificity of 75%
Assay principles: This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Brucella bovine or sheep IgG in serum. The kit consists of high-bind pre-coated, detachable 96-well plate, Positive control, Negative Control, an HRP-Anti-bovine or sheep IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37 °C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml).
2. Dilute the secondary HRP antibody by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5 to 1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5 to 30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD value reflects the amount of antibody bound.
Storage and shipping condition: Shipment with room temperature. The unopened kit is stable for 6 months from the date of manufacture when stored at 2 to 8 °C. Avoid light. The shelf life is 60 days from the date of opening.
