miniPCR® Sleep Lab™ – Lark or Owl?

Supplier: AMPLYUS LLC

MINIPCR sleep lab

MINIPCR sleep lab

MINIPCR sleep lab

MINIPCR sleep lab

miniPCR®

KT-1005-01

470352-052EA 151.49 CAD

470352-052

miniPCR® Sleep Lab™ – Lark or Owl?

Educational Classroom Kits and Activities

Participate in an authentic investigation on the genetics of sleep and circadian clocks.

Techniques: Micropipetting, DNA extraction, PCR, gel electrophoresis
Topics: Circadian rhythms, genetic inheritance, genotype to phenotype, genetic associations, biotechnology
Time required: Can be completed in two 45-minute class periods or one 120-minute class period
Level: Advanced high school through college

Students will test their own genotypes at the circadian clock gene Per3 that has been associated with sleep behavior preferences in humans. This gene’s coding region contains a VNTR (variable number tandem repeat) that is polymorphic (variable) across individuals. Different variants of this VNTR in Per3 have been associated with a preference for evening or morning activity–whether you are a morning or evening type. Students will assess their own Per3 genotypes, while also assessing their sleep phenotypes through a simple circadian questionnaire, with the ultimate goal of learning about circadian clocks and the study of genetic associations. Participate in an authentic open inquiry investigation on the genetic underpinnings of sleep.

Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes

Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and visualization system

Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer

Accessories information: The learning lab companion kit (catalog no. 470352-026) contains all the consumables (except water) needed for a class of 32 students. These reagents and consumables can also be sourced independently.

Delivery information: The miniPCR Sleep Lab™ kit contains reagents for 8 lab groups of 4 students each (32 students): X-Tract™ DNA Extraction Buffer (2 vials); 5X EZ PCR Master Mix, Load-Ready™; Sleep Lab Primer Mix; 100 bp DNA Ladder, Load Ready™; Note: Reagents are compatible with blueGel™ or other standard horizontal electrophoresis systems.

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