Human Aldehyde Oxidase/AOX1 ELISA Kit

Supplier: Antibodies.com LLC
A77691-96
76730-610EA 984.58 CAD
76730-610
Human Aldehyde Oxidase/AOX1 ELISA Kit
Assays ELISAs
Human Aldehyde Oxidase/AOX1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Aldehyde Oxidase/AOX1 in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 0.625 to 40 ng/ml
  • Sensitivity: 0.375 ng/ml
  • Sample type: Serum, plasma, tissue homogenates and other biological fluids
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human Aldehyde Oxidase/AOX1 ELISA kit (A77691) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Aldehyde Oxidase/AOX1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Aldehyde Oxidase/AOX1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aldehyde Oxidase/AOX1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Aldehyde Oxidase/AOX1 Antibody, which binds the captured Aldehyde Oxidase/AOX1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Aldehyde Oxidase/AOX1 captured in each well. The concentration of Aldehyde Oxidase/AOX1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.
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