Betaine enhancer solution 5M

Supplier: VWR International

Synonyms: 2-(Trimethylammonio)acetate , Trimethylglycine , TMG

V351104

76620-530EA 104.77 CAD

76620-530

Betaine enhancer solution 5M

Betaine

Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature.

Enhances amplification on GC-rich and difficult DNA targets
Lowers the melting temperature

The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.

Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.

Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₅H₁₁NO₂
MW: 117.15 g/mol
Storage Temperature: Freezer

MDL Number: MFCD00012123
CAS Number: 107-43-7

Specification Test Results

Functional test - 1X Betaine Enchancer Solution is tested for amplification of 613 bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions	Passes test
Volume check- fill volume >= 0.1 ml per tube	Passes test

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