TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use mix offering easy reaction assembly at room temperature prior to PCR. Just add your template and primers to carry out PCR.
- Minimal optimization
- Reaction set up at room temperature
- Time-saving
- Increased sensitivity, specificity and product yield
- Processes up to 4 kb
- Detection of low abundance targets
- Consistent test results
- Lower risk of contamination
TEMPase Hot Start DNA Polymerase is suitable for detection of low abundance targets, screening, multiplexing, direct colony PCR and Real time PCR applications.
TEMPase Hot Start DNA Polymerase Master Mix is a ready to use 2X master mix composed of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl2 and an optimized buffer system. TEMPase Hot Start DNA Polymerase has been designed to diminish the formation of non-specific priming events during reaction set-up and the first ramp of thermal cycling.
The blue loading dye in Blue TEMPase Hot Start DNA Polymerase 2x Master Mix facilitates direct gel loading, and eliminates the need for separate loading dye - no need for time-consuming sample preparation before electrophoresis.
Multiplex 2× Master Mix is composed of TEMPase Hot Start DNA Polymerase and a specialized buffer system designed for multiplex PCR.
Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are available in two variations, either based on Key Buffer (Master Mix K) or Combination Buffer (Master Mix C) to suit different PCR requirements. Additional MgCl₂ is included with the Multiplex Tempase Hot Start Master Mix to enable optimization. The final concentration of MgCl₂ is indicated in brackets.
Caution: For Research Use Only. Not for use in diagnostic procedures.
