VWR® TAQ DNA Polymerase

Supplier: VWR
VWR®
V114103 V114107 V114404 V114102 V114106V V111204 V114104 V110025 V114108 V111206V V114408 V111208 V112504
76620-428EA 637.06 CAD
76620-428 76620-438 76620-426 76620-448 76620-436 76620-446 76620-424 76620-434 76620-444 76620-432 76620-442 76620-430 76620-440
VWR® TAQ DNA Polymerase
Nucleic Acid Reagents End-point PCR Enzymes and Kits
VWR® Taq DNA polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimization.

  • A thermostable recombinant DNA polymerase
  • High product yield
  • dUTP incorporation possible
  • Processes up to 5 kb
  • Leaves a 3'dA overhang

Taq DNA polymerase concentration: 5 Units/μl

10X Key Buffer: Tris-HCl pH 8.5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8.3; KCl, 15 mM MgCl₂, 1% Tween®20
10X Key Buffer (Mg²⁺ free): Tris-HCl pH 8.5; (NH₄)₂SO₂, 1% Tween® 20
10X Extra Buffer (Mg²⁺ free): Tris-HCl pH 8.3; KCl, 1% Tween®20

Taq DNA polymerase is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). It leaves an A' overhang, which makes the enzyme ideal for TA cloning.

Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high-throughput testing.

Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.

Additional MgCl₂ is included with each kit to enable optimization.

Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimizing the need for optimization of the Mg²⁺ concentration or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimization of primer annealing temperatures and Mg²⁺ concentrations may be required.

Taq DNA polymerase is also available in a glycerol-free format for automation and lyophilization.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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