L-Photo-Methionine, Pierce™
Supplier: Thermo Scientific
Synonyms:
Photo-Methionine
, L-2-amino-5,5'-azi-hexanoic acid
Thermo Scientific Pierce L-Photo-Methionine is an analog of L-methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.
- In vivo labeling—incorporate photo-reactive group into proteins using normal cellular machinery of live cells
- In vivo crosslinking—find interacting proteins in the native cellular environment
- Increased specificity—crosslink interacting proteins correctly positioned at their interfaces within protein interaction domains
- Efficient recovery—90% protein recovery in cell lysates after crosslinking
- Compatible—crosslink proteins expressed in a wide variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3 and C6
- Easy to use—reagents are photo-stable under normal laboratory lighting, eliminating the need to work in the dark
Thermo Scientific L-Photo-Methionine is an analog of L-methionine that can be endogenously incorporated into the primary sequence of proteins during synthesis and then ultraviolet light (UV)-activated to covalently crosslink proteins within protein-protein interaction domains in their native environment within live cells. The powerful method enables both stable and transient protein interactions in cells to be studied and characterized without the use of completely foreign chemical crosslinkers and associated reagent solvents during the interaction experiment that can adversely aspects of the cell biology being studied.
Caution: For Research Use Only. Not for use in diagnostic procedures.
Specification Test Results
Product Line | Pierce™ |
Labeling Method | Chemical Labeling, Metabolic Labeling |
Crosslinker Type | Heterobifunctional |
Reactive Moiety | Diazirine |
Spacer | Short |
Form | Solid |
Quantity | 100 mg |
Type | Crosslinker |
Solubility | Water |
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