ZORBAX GF-250/450 Kits, Agilent Technologies

Supplier: Agilent
820400-901 420420-901 884973-701 884973-761 884973-761 884973-701 820212-911 820444-901 820444-901 820999-901 820212-911 884973-902 820400-901 820950-911 840140-901 840140-901 884973-902 820950-911 820999-901 420420-901
CA840140-901EA 609.47 CAD
AG884973-701 CA840140-901 AG820400-901 AG884973-902 CAAG820212-911 AG420420-901 CAAG884973-761 CA420420-901 CA820950-911 AG820950-911 CAAG884973-701 AG820444-901 AG840140-901 AG820212-911 CA820400-901 CA820999-901 AG820999-901 CA884973-902 AG884973-761 CAAG820444-901
ZORBAX GF-250/450 Kits, Agilent Technologies
Chromatography Columns
Legacy zirconium-stabilized silica diol size exclusion columns (USP designation L35).

  • High efficiency and reproducibility with short analysis time
  • Compatible with organic modifiers and denaturants
  • Semi-prep and prep column dimensions
  • Wide usable pH range (3-8)

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4000-900000 for globular proteins when using GF-250 and GF-450 columns in series.

The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.

The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.

Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.
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