The Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on nitrocellulose membranes after transfer from polyacrylamide gels.
- Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
- Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
- Protocol—stain in less than five minutes; completely erase and reverse staining in less than 15 minutes
- Stability—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps
This kit for membrane staining uses a nondestructive, reversible, reliable, and sensitive method to stain and detect proteins on nitrocellulose membranes. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents. The staining protocol is simple and quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane..
Contents
Sufficient For: 10 nitrocellulose membranes (8 cm x 8 cm) from mini gels (SDS-PAGE)
• Reversible Stain, 250 mL
• Destain, 2 x 500 mL
• Stain Eraser, 250 mL
Caution: Store at room temperature
