NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), New England Biolabs

Supplier: New England Biolabs

NEBNext ARTIC SARS-CoV-2 FS library prep kit

NEBNext ARTIC SARS-CoV-2 FS library prep kit

NEBNext®

E7658S E7658L E7658S E7658L

CA76474-310EA 603 CAD

76474-310 76474-312 CA76474-310 CA76474-312

NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), New England Biolabs

Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing SARS-CoV-2.

Updated primers for effective Omicron coverage
Express protocol option, with fewer cleanups
Library inserts in the 150 bp range
Compatible with 2×75 Illumina sequencing

These kits, for long and short read sequencing, were based on the original work of the ARTIC Network. The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach. NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation. The balanced primers and reagents for RT-PCR deliver improved uniformity of amplicon yields from gRNA across a wide copy number range. Library preparation uses the NEBNext FS reagents and workflow, incorporating enzymatic cDNA fragmentation, and with reagent volumes optimized for the ARTIC application. Required adaptors and primers are purchased separately. The novel NEBNext Library PCR Master Mix allows use of the same number of PCR cycles to amplify libraries, regardless of initial input amounts.

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