NEBExpress Ni-NTA Magnetic Beads, New England Biolabs

Supplier: New England Biolabs
S1423S S1423S S1423L S1423L
CA76339-766EA 1250 CAD
76339-766 76339-768 CA76339-766 CA76339-768
NEBExpress Ni-NTA Magnetic Beads, New England Biolabs
Beads Magnetic Beads
An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats. Immobilized Metal Affinity Chromatography (IMAC) purifications employing Ni-NTA (nickel-nitrilotriacetic acid) magnetic beads can be performed under native or denaturing conditions which permit efficient binding and purification of insoluble proteins, proteins that aggregate in inclusion bodies, or proteins that possess a tertiary structure that sequester the polyhistidine affinity tag.

  • NTA coordination exhibits low Nickel ion leaching
  • Tolerates a wide range of conditions, including the presence of protein denaturants and detergents
  • Low non-specific binding permits immobilized fusion proteins to be used in subsequent experiments
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