Q5U Hot Start High-Fidelity DNA Polymerase, New England Biolabs

Supplier: New England Biolabs (NEB)

M0515L M0515S
CA76339-738EA 1119 CAD
CA76339-738 CA76339-740
Q5U Hot Start High-Fidelity DNA Polymerase, New England Biolabs
Nucleic Acid Reagents End-point PCR Enzymes and Kits

Q5U Hot Start high-fidelity DNA Polymerase is a modified version of Q5 high-fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain.


  • Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (FFPE)
  • Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
  • Generate higher assembly efficiency and improve accuracy in USER cloning
  • Enable room temperature setup with aptamer-based hot start formulation


Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

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