Quick CIP

Supplier: New England Biolabs

M0525S M0525L M0525S M0525L

CA76339-742EA 693 CAD

76339-742 76339-744 CA76339-742 CA76339-744

Quick CIP

Enzymes

Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs).

Rapid and irreversible heat inactivation eliminates unwanted activity
Improved storage stability versus native enzyme
Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)

Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5 protruding, 5 recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.

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