Template Switching RT Enzyme Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

Template switching RT enzyme mix

Template switching RT enzyme mix

M0466L M0466S

CA76339-734EA 616 CAD

CA76339-734 CA76339-736

Template Switching RT Enzyme Mix, New England Biolabs

Protein Linking and Labeling Systems

The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.

Prepare RNA-seq libraries from extremely low input: single cells/nuclei or 2 pg of total RNA
Perform 5' RACE from as low as 10 ng total RNA
Low background for RNA-seq or 5' RACE
Enzyme mix contains Murine RNase Inhibitor, no additives necessary

These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.

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