EMS SmartBlue™ Blue light transilluminator replaces the old method of nucleic acid detection.
- Blue light, safe for nucleic acids
- Uniform illumination (UI Technology)
- Optimal wavelength (465 nm) for green stains
- Easily removable, two-position amber shield
- Can be used with SmartDoc™ for gel imaging with a smart phone
New fluorophores have been developed to detect nucleic acid with toxic ethidium bromide. They also eliminate the use of DNA damaging UV light, as they are visualized with blue light. The SmartBlue™ Transilluminator is the perfect partner for these new stains. It emits blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophores, as well as many protein stains. Exposing DNA to this wavelength does not cause damage and results in a much higher down stream cloning efficiency as compared to even short UV light exposures. In addition, the blue light is not damaging to the skin and eyes.
The SmartBlue™ Transilluminator requires no special shielding; however, the light does need to be filtered for visualization of the dyes. The amber cover, that rest above the viewing surface and gel, filters out the blue light, allowing the bands to be seen clearly. The cover can be placed at a 60° angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely.
Engineered diffusers and filters provide an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.
An array of super bright LEDs with a long, 30000 hour service life provide the light source for the SmartBlue™ Transilluminator. The filters in the SmartBlue™ will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surface. To save energy, the power switch includes an automatic five minute shutoff.