Monarch® HMW DNA Extraction Kits for Tissue, New England Biolabs

Supplier: New England Biolabs (NEB)

Monarch® HMW DNA extraction kits for tissue

Monarch® HMW DNA extraction kits for tissue

T3060S T3060L

CA76470-750EA 136 CAD

CA76470-750 CA76470-752

Monarch® HMW DNA Extraction Kits for Tissue, New England Biolabs

Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents

The Monarch® HMW DNA extraction kit for tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian.

Isolate DNA into the Mb size range from soft organ tissues and bacteria
Fast, user-friendly workflows (90 minutes for tissues and bacteria)
Obtain excellent yields of highly-pure and intact DNA; ideal for long read sequencing
Includes RNase A and Proteinase K, as well as microtube pestles for convenient sample homogenization

The optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to ≥500 kb for the standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For tissue and bacteria, the processing time is 90 min. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing.

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