PaqCI, New England Biolabs

Supplier: New England Biolabs (NEB)

PaqCI, New England Biolabs

PaqCI, New England Biolabs

R0745S R0745L

CA76470-740EA 165 CAD

CA76470-740 CA76470-742

PaqCI, New England Biolabs

Nucleic Acid Reagents Restriction Enzymes

PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(⁴/₈).

Useful in Golden gate assembly - 7 bp cutter leads to less domestication problems
PaqCI is an isoschizomer of AarI that effectively cuts to completion (unlike AarI)
Requires 2 or more sites for cleavage. PaqCI Activator supplied to improve cleavage

It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's CutSmart buffer and is heat inactivated.

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