NEBNext® Enzymatic Methyl-seq (EM-seq™) Conversion Module, New England Biolabs

Supplier: New England Biolabs
E7125S E7125S E7125L E7125L
CA76339-722EA 989 CAD
76339-722 76339-724 CA76339-722 CA76339-724
NEBNext® Enzymatic Methyl-seq (EM-seq™) Conversion Module, New England Biolabs
Microbiology Test Systems Enzymatic Tests
NEBNext® Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

  • High efficiency conversion
  • Minimal DNA damage
  • Minimized GC bias

While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. The NEBNext® Enzymatic Methyl-seq (EM-seq™) conversion module provides an enzyme-based alternative to bisulfite conversion.

For use in Illumina® sequencing workflows, we recommend can use of the NEBNext® Enzymatic Methyl-seq (EM-seq™) kit (76337-184, 76337-038) instead of this module. The full kit includes the EM-seq™ Adaptor, which has been optimized for high performance in the EM-seq™ workflow, as well as the high-efficiency Ultra II library prep reagents, and these components are not included in the conversion module.
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