NEBNext® Enzymatic Methyl-seq (EM-seq™) Kit, New England Biolabs

Supplier: New England Biolabs

NEBNext® Enzymatic methyl-seq (EM-seq™) kit

NEBNext® Enzymatic methyl-seq (EM-seq™) kit

E7120S E7120L E7120L E7120S

CA76337-038EA 4921 CAD

76337-038 CA76337-038 76337-184 CA76337-184

NEBNext® Enzymatic Methyl-seq (EM-seq™) Kit, New England Biolabs

Microbiology Test Systems Enzymatic Tests

NEBNext® Enzymatic methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

Superior sensitivity of detection of 5-mC and 5-hmC
Greater mapping efficiency
More uniform GC coverage
Detection of more CpGs with fewer sequence reads
Uniform dinucleotide distribution
High-efficiency library preparation, with larger library insert sizes
Conversion module also available separately

While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. In contrast, the highly effective enzyme-based conversion in the NEBNext® Enzymatic Methyl-seq (EM-seq™) kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.

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