NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3), New England Biolabs

Supplier: New England Biolabs (NEB)

E6444L E6444S
CA76447-120EA 3117 CAD
CA76447-120 CA76447-122
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3), New England Biolabs
Nucleic Acid Reagents RT-PCR and cDNA Reagents

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production. 


  • Enables complete filtering of index-hopped reads
  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 2, and 4 for 384 barcodes


The NEBNext Adaptor has a unique hairpin loop structure that minimizes adaptor-dimer formation, while NEBNext index PCR primers enable index (barcode) incorporation during library amplification. This third set of unique-dual indices (UDIs) includes 96 pre-mixed unique pairs of index primers (i5 and i7), packaged in a single-use 96-well plate with a pierceable foil seal.


Additional NEBNext Multiplex Oligos products are available in sets of 12 and 96 single index primers as well as additional dual index primers for increased sample identification specificity.

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