QuantiChrom™ Carbonyl Assay Kit, BioAssay Systems

Supplier: BioAssay Systems

Carbonyl assay graph

Carbonyl assay graph

DCAR-100

76423-318EA 706.51 CAD

76423-318

QuantiChrom™ Carbonyl Assay Kit, BioAssay Systems

Assays Protein Assays

Quantification of carbonyl groups or protein carbonyls in biological samples.

Fast and sensitive: use of 100 µl sample, linear detection range from 12 to 250 µm carbonyl in 96-well plate assay
The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes
Detection limit: 12 µm
Method: OD375nm
Samples: chemicals with carbonyl groups or protein carbonyls in biological samples

Carbonyl groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' carbonyl assay kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.

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