HiTrap MabSelect SuRe PCC chromatography columns are prepacked with MabSelect SuRe™ pcc protein A resin for screening and small-scale mAb purification in continuous bioprocessing using periodic counter-current chromatography (PCC).
- Convenient prepacked column for fast, reproducible, and easy small-scale purifications
- High binding capacity, ~60 mg human IgG/ml resin at 2.4 min residence time
- High productivity through capture of large mass of monoclonal antibodies in a short period of time
- Highly concentrated elution pools for operating flexibility and small unit operations
- Allows cost-effective cleaning with 0.1 to 0.5 M NaOH over hundreds of purification cycles
- High protein ligand stability reduces ligand leakage
HiTrap MabSelect SuRe PCC packed columns (1 and 5 ml) are suited for small scale antibody purifications, as well as optimization of methods and parameters, such as sample load and protein binding conditions. These protein A columns provide fast, reproducible, and easy separations in a convenient format. The columns are optimally utilized when they are connected to liquid chromatography systems such as the AKTA systems.
The alkali-stabilised, protein A-derived ligand gives the resin greater stability than conventional protein A-based resins under the alkaline conditions used in cleaning-in-place (CIP) protocols. Consequently, the possibility of cleaning with cost-effective reagents such as sodium hydroxide (NaOH) will improve process economy.
In addition, the use of NaOH for cleaning efficiently inactivates bacteria, mold, and yeast, with improved product quality as a result.
