The Biosensis NGFR/p75ECD RapidTM ELISA kit is validated for precise and accurate quantification of urinary p75, a biomarker of ALS/MND.
- Complete kit with precise instructions
- Assay duration of less than four hours without compromising quality
- Accurate quantification of p75ECD in human urine samples
The Biosensis NGFR/p75ECD RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application.
This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a biotinylated mouse monoclonal anti-p75ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. A human p75ECD positive control (QC sample) is provided to assure consistent assay performance.
This NGFR/p75ECD ELISA kit employs a recombinant human p75ECD-Fc chimera. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75ECD as p75ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75ECD.
Ordering information: Previous customers of BEK-2219 please note: The capture and detection antibodies have been optimized for BEK-2239. The new monoclonal antibody pair yields higher OD values in urine samples, demonstrating its increased ability to detect natural p75ECD in biological samples. This means that samples assayed with BEK-2239 will give higher urine p75ECD levels, or that urine samples from healthy controls with previous urine p75ECD concentrations <LOD may read positive. Also, BEK-2239 includes a standard obtained from a different source, providing a reference standard curve different to BEK-2219.
Delivery information: The ELISA kit box contains 1 or 2×96-well pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Caution: This kit has been configured for research use only and is not to be used for diagnostic or clinical procedures.
